THE EFFECT OF TEMPERATURE AND PHOTOPERIOD ON BIOREMEDIATION POTENTIALS OF MONO AND MIXED CULTURES OF CHLOROPHYTES AND CYANOBACTERIA FOR MUNICIPAL WASTEWATER TREATMENT

Abstract

Investigation on organic xenobiotics bioaccumulation/biodegradation in green algae is of great importance from environmental point of view because widespread distribution of these compounds in agricultural areas has become one of the major problems in aquatic ecosystem. Temperature, light intensity, amount and type of nutrients, amount of CO2, and pH are the key factors influencing algal growth. Collections of Wastewater Samples were done according standard method. BG-11 media was prepared for culture of microalgae. Identification and isolation of microalgae was done according standard method and haemocytometer were used for microalgae cell counting. The results indicated that temperature and photoperiod affect the activities of microalgae; temperature at 25°C provide with 12:12h light/dark photoperiods show more significant with increases the number of days for both of the species as well as mixed culture, when compared with the treatments at temperature of 10°C provide with 6/18h light/dark photoperiods. Furthermore, Chlorogonium sp. and Chlorella sorokiniana showed more response during treatments in increase with the number of weeks when compared with Microcystis earuginosa and mixed culture of the species. Therefore, temperature and photoperiod impacts potentials of microalgae in the remediation of wastewater effluents.

Key Words:Temperature, Photoperiod, Bioremediation, Wastewater and Algal Culture

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